NIA Array Analysis Tool
HELP:
General description of NIA Array Analysis Tool
Arrayjoin Tool
(Windows) for building the input file from multiple scanner files (
DOWNLOAD
)
Upload Data File:
see
file format
Normalize
Split-normalize
upload as:
Data name (use 1-word; if blank, = filename)
None
Log10
Log2
Loge
Log-transformation in input file
Computer guess
Use headers
Don't use headers
No replications 1-color
No replications 2-color
Lower cutoff value
GNF_SymAtlas_mouse
Activated_Dormant_Blastocyst
MixingExpt
AbundanceEstim
mouse_preimplantation
ESC_EGC_LIFplus_Fig3
ESC_EGC_LIFplus_LIFminus_RA_Fig6
ESC_EGC_TS_NSC_Fig2
BAF250
GonadAging
Sox2_KO__Sharov_2008
Oct4_KO_44k_Sharov_2008
Oct4_KO_22k_Sharov_2008
Nanog_overExpr_Sharov_2008
Rex1_EB5
mRNA_degradation_rate
linear_trajectory_Fig3
lineage_trajectories_Fig1
ES_bank_time_course
Public pages
Select data file for analysis
demo
Description:
(none)
Universal
Universal NIA October 2008 Best Oligo
Agilent 22K oligo array, ver1
Agilent 22K commercial
xenopus
rat
agilent human
GNF mouse atlas ver2
Mouse 430 2.0
xenopus2
deac
HG-U133A
Agilent whole-genome mouse 44k
Universal NIA oligo 01-2006
Illumina mouse
Universal NIA January 2007
Universal NIA January 2008
Universal NIA January 2008-Best Oligo
NIA 15K membrane
NIA 15K old
MEEBO_MI_051705
HEEBO V1.01
Xenopus 44k Custom Array Annotation 09-08-2008
Illumina Human 48K
Illumina Human 48K II
HOA Onearray
Illumina Human Ref8 v2
Illumina Human Ref-8 v3
Illumina Mouse WG-6v2
Array annotation
3
4
6
8
10
15
10000
Threshold z-value to remove outliers
Actual
Average
Bayesian
Max(Average,Actual)
Max(Average,Bayesian)
Error model
50
100
200
500
1000
2000
5000
10000
Size of sliding window for
averaging error variances
0
0.01
0.05
0.1
Proportion of highest variance values to be removed before
variance averaging
5
10
20
50
100
Desirable degrees of freedom for
Bayesian
error model
0.01
0.05
0.1
0.2
0.5
FDR
false discovery rate threshold
0
100
200
500
1000
2000
5000
Number of
permutations
1-color arrays
2-color arrays
Number of colors in the
array
Minimum log-intensity
cutoff
(-9999 = none)
Maximum log-intensity
cutoff
(-9999 = none)
don't analyze reference
analyze reference
Include
reference
into ANOVA analysis
Reference
name
No
cross-channel correction
Table of tissues/experiments (see
help
)
N data columns=
#
No. of
re-
plications
Name of tissue/experiment
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