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Detailed Description of Mouse cDNA Libraries |
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| Name | Mouse Undifferentiated ES Cell cDNA Library (Long) |
| Organism | Mus musculus |
| Strain | 129/Sv x 129/Sv-CP |
| Sex | Unknown |
| Stage | R1 ES cells |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]).Total RNAs were obtained from Dr. Kenneth R. Boheler (National Institute on Aging, USA). ES cells were cultured without feeder cells in the presence of LIF and BRL-conditioned media. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 14.2 µg of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.4 kb. The library was constructed by Yulan Piao. |
| Name | Mouse Undifferentiated ES Cell cDNA Library (Short) |
| Organism | Mus musculus |
| Strain | 129/Sv x 129/Sv-CP |
| Sex | Unknown |
| Stage | R1 ES cells |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| This is a short-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]).Total RNAs were obtained from Dr. Kenneth R. Boheler (National Institute on Aging, USA). ES cells were cultured without feeder cells in the presence of LIF and BRL-conditioned media. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 14.2 µg of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-L. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 1.4 kb. The library was constructed by Yulan Piao. |
| Name | Mouse ES Cell (LIF-) cDNA Library (Long) |
| Organism | Mus musculus |
| Strain | 129/Sv x 129/Sv-CP |
| Sex | Unknown |
| Stage | R1 ES cells |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]).Total RNAs were obtained from Dr. Kenneth R. Boheler (National Institute on Aging, USA). ES cells were cultured without feeder cells in the absence of LIF for 4 hours or 18 hours. Equimolar mixture of these RNA samples was used for the library construction. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 21 µg of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.4 kb. The library was constructed by Yulan Piao. |
| Name | Mouse ES Cell (LIF-) cDNA Library (Short) |
| Organism | Mus musculus |
| Strain | 129/Sv x 129/Sv-CP |
| Sex | Unknown |
| Stage | R1 ES cells |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| This is a short-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]).Total RNAs were obtained from Dr. Kenneth R. Boheler (National Institute on Aging, USA). ES cells were cultured without feeder cells in the absence of LIF for 4 hours or 18 hours. Equimolar mixture of these RNA samples was used for the library construction. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 21 µg of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-L. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 1.3 kb. The library was constructed by Yulan Piao. |
| Name | Mouse Trophoblast Stem Cell cDNA Library (Long) |
| Organism | Mus musculus |
| Strain | B5/EGFP transgenic ICR mice |
| Sex | Unknown |
| Stage | 3.5-dpc |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]).Total RNAs were obtained from Dr. Janet Rossant and Tilo Kunath (Samuel Lunenfeld Research Institute, Canada). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen:5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 4 µg of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.6 kb. The library was constructed by Yulan Piao. |
| Name | Mouse Trophoblast Stem Cell cDNA Library (Short) |
| Organism | Mus musculus |
| Strain | B5/EGFP transgenic ICR mice |
| Sex | Unknown |
| Stage | 3.5-dpc |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| This is a short-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]).Total RNAs were obtained from Dr. Janet Rossant and Tilo Kunath (Samuel Lunenfeld Research Institute, Canada). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 4 µg of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-L. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 1.3 kb. The library was constructed by Yulan Piao. |
| Name | Mouse Hematopoietic Stem Cell (Lin-/c-Kit+/Sca-1-) cDNA Library (Long) |
| Organism | Mus musculus |
| Strain | C57BL/6NCr |
| Sex | Unknown |
| Stage | Age ~10 weeks old |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]).Total RNAs were obtained from Drs. Dennis Taub, Dan Longo (National Institute on Aging, USA), Jonathan Keller (National Cancer Institute, USA). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 2.4 µg of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.2 kb. The library was constructed by Yulan Piao. |
| Name | Mouse Hematopoietic Stem Cell (Lin-/c-Kit+/Sca-1+) cDNA Library (Long) |
| Organism | Mus musculus |
| Strain | C57BL/6NCr |
| Sex | Unknown |
| Stage | Age ~10 weeks old |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]).Total RNAs were obtained from Drs. Dennis Taub, Dan Longo (National Institute on Aging, USA), Jonathan Keller (National Cancer Institute, USA). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 4.8 µg of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.7 kb. The library was constructed by Yulan Piao. |
| Name | Mouse Hematopoietic Stem Cell (Lin-/c-Kit-/Sca-1+) cDNA Library (Long) |
| Organism | Mus musculus |
| Strain | C57BL/6NCr |
| Sex | Unknown |
| Stage | Age ~10 weeks old |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]).Total RNAs were obtained from Drs. Dennis Taub, Dan Longo (National Institute on Aging, USA), Jonathan Keller (National Cancer Institute, USA). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 1.1 µg of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.2 kb. The library was constructed by Yulan Piao. |
| Name | Mouse Hematopoietic Stem Cell (Lin-/c-Kit-/Sca-1-) cDNA Library (Long) |
| Organism | Mus musculus |
| Strain | C57BL/6NCr |
| Sex | Unknown |
| Stage | Age ~10 weeks old |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]).Total RNAs were obtained from Drs. Dennis Taub, Dan Longo (National Institute on Aging, USA), Jonathan Keller (National Cancer Institute, USA). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 0.9 µg of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.1 kb. The library was constructed by Yulan Piao. |
| Name | Mouse Osteoblast cDNA Library (Long) |
| Organism | Mus musculus |
| Strain | C3H/He mice |
| Sex | Unknown |
| Stage | KUSA/A1 cells |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]).Total RNAs were obtained from Dr. Akihiro Umezawa (Keio University School of Medicine, Japan). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 2.1 µg of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 3.0 kb. The library was constructed by Yulan Piao. |
| Name | Mouse Mesenchymal Stem Cell cDNA Library (Long) |
| Organism | Mus musculus |
| Strain | C3H/He mice |
| Sex | Unknown |
| Stage | 9-15C cells |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]).Total RNAs were obtained from Dr. Akihiro Umezawa (Keio University School of Medicine, Japan). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 2.2 µg of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.5 kb. The library was constructed by Yulan Piao. |
| Name | Mouse Blastocyst cDNA Library (Long) |
| Organism | Mus musculus |
| Strain | C57BL/6J |
| Sex | Unknown |
| Stage | 3.5-dpc |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]).Total RNAs were extracted from a pool of 20 Blastocysts. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 0.2 µg of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.2 kb. The library was constructed by Yulan Piao. |
| Name | Mouse 7.5-dpc Whole Embryo cDNA Library (Long) |
| Organism | Mus musculus |
| Strain | C57BL/6J |
| Sex | Unknown |
| Stage | 7.5-dpc |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]).Total RNAs were extracted from a pool of four embryos at 7.5-days postcoitum. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 7 µg of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.2 kb. The library was constructed by Yulan Piao. |
| Name | Mouse Unfertilized Egg cDNA Library (Long) |
| Organism | Mus musculus |
| Strain | C57BL/6J |
| Sex | Unknown |
| Stage | Unfertilized Egg |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]).Total RNAs were extracted from a pool of 1488 unfertilized eggs. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'], treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.5 kb. The library was constructed by Yulan Piao. |
| Name | Mouse 8.5-dpc Whole Embryo cDNA Library (Long) |
| Organism | Mus musculus |
| Strain | C57BL/6J |
| Sex | Unknown |
| Stage | Whole embryo including extraembryonic tissues at 8.5-days postcoitum |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]).Total RNAs were extracted from a pool of 13 embryos at 8.5-days postcoitum. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 9.1 µg of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.5 kb. The library was constructed by Yulan Piao. |
| Name | Mouse 12.5-dpc Male Genital Ridge/Mesonephros cDNA Library (Long) |
| Organism | Mus musculus |
| Strain | C57BL/6J |
| Sex | Male |
| Stage | 12.5-dpc |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]).Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 1.8 µg of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.4 kb. The library was constructed by Yulan Piao. |
| Name | Mouse Newborn Heart cDNA Library |
| Organism | Mus musculus |
| Strain | C57BL/6J |
| Sex | Unknown |
| Stage | Newborn Heart |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 24.9 µg of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal3 (Ref. Development 127: 1737-1749 (2000)PMID:10725249]),purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were digested with SalI and NotI enzymes, and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the chemical method. The average insert size is about 1.8 kb. The library was constructed by Yulan Piao. |
| Name | Mouse Newborn Brain cDNA Library |
| Organism | Mus musculus |
| Strain | C57BL/6J |
| Sex | Unknown |
| Stage | Newborn Brain |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 48µg of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal3 (Ref. Development 127: 1737-1749 (2000) PMID:10725249]),purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were digested with SalI and NotI enzymes, and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 1.9 kb. The library was constructed by Yulan Piao. |
| Name | Newborn Kidney cDNA Library (Long) |
| Organism | Mus musculus |
| Strain | C57BL/6J |
| Sex | Unknown |
| Stage | Newborn Kidney |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). In brief,Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 26 µg of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 3.0 kb. The library was constructed by Yulan Piao. |
| Name | Newborn Kidney cDNA Library (Short) |
| Organism | Mus musculus |
| Strain | C57BL/6J |
| Sex | Unknown |
| Stage | Newborn Kidney |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| This is a short-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). In brief, double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 26 µg of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-L. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 1.5 kb. The library was constructed by Yulan Piao. |
| Name | Mouse Germinal Center B Cell cDNA Library |
| Organism | Mus musculus |
| Strain | |
| Sex | Unknown |
| Stage | |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| FACS-sorted Germinal Center B cells were provided by Drs. Richard Hodes, Emily Klotz (National Institute on Aging and National Cancer Institute, USA) and Garnett Kelsoe (Duke University, USA). Double-stranded cDNAs were synthesized from 0.46 µg of total RNA with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'], treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal3 (Ref. Development 127: 1737-1749 (2000) [PMID: 10725249]), purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) and purified by phenol/chloroform, followed by Centricon 100 purification. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is 1.2 kb. The library was constructed by Yulan Piao. |
| Name | NIA Mouse E13.5 VMB Dopamine cell cDNA Library |
| Organism | Mus musculus |
| Strain | TH-beta-gal transgenic mouse |
| Sex | Unknown |
| Stage | 13.5-dpc |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were extracted from a pool of 3000 dopamine cells by Drs. Tanya Barrett and Dave Donovan (National Institute on Aging, USA). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 0.9 µg of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.0 kb. The library was constructed by Yulan Piao. |
| Name | Mouse unfertilized egg cDNA |
| Organism | Mus musculus |
| Strain | C57BL/6J |
| Sex | Unknown |
| Stage | Unfertilized Egg |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| Total RNAs were extracted from 1528 unfertilized egg. The double-stranded cDNA was synthesized by Gibco's kit with an Oligo(dT) primer [NotI primer-adapter from GibcoBRL] [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 2.7µg of total RNA . The double-stranded cDNAs were treated with T4 DNA polymerase and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal3 (include Sal1 sequence). The cDNAs were purified by phenol/chloroform and separated from free linkers by Centricon 100. Then, cDNAs were amplified by long-range high fidelity PCR using Takara's Ex Taq polymerase. Then, the cDNAs were purified by phenol/chloroform and by Centricon 100. The cDNAs were digested with SalI and NotI enzymes. Then, the cDNAs were size selected by Gibco's Size Fractionation Column. The cDNAs were cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by chemical method.The library was constructed by Xiaohong Wang and Minoru S. H. Ko. |
| Name | Mouse fertilized one-cell-embryo cDNA |
| Organism | Mus musculus |
| Strain | C57BL/6J |
| Sex | Unknown |
| Stage | Fertilized Egg |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| Total RNAs were extracted from 1137 fertilized eggs. The double-stranded cDNA was synthesized by Gibco's kit with an Oligo(dT) primer [NotI primer-adapter from GibcoBRL] [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 5.4µg of total RNA . The double-stranded cDNAs were treated with T4 DNA polymerase and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal3 (include Sal1 sequence). The cDNAs were purified by phenol/chloroform and separated from free linkers by Centricon 100. Then, cDNAs were amplified by long-range high fidelity PCR using Takara's Ex Taq polymerase. Then, the cDNAs were purified by phenol/chloroform and by Centricon 100. The cDNAs were digested with SalI and NotI enzymes. Then, the cDNAs were size selected by Gibco's Size Fractionation Column. The cDNAs were cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by chemical method. The library was constructed by Xiaohong Wang and Minoru S. H. Ko. |
| Name | Mouse two-cell stage embryo cDNA |
| Organism | Mus musculus |
| Strain | C57BL/6J |
| Sex | Unknown |
| Stage | 2-cell stage embryo |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| Total RNAs were extracted from 397 two-cell stage embryos. The double-stranded cDNA was synthesized by Gibco's kit with an Oligo(dT) primer [NotI primer-adapter from GibcoBRL] [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 1.2µg of total RNA . The double-stranded cDNAs were treated with T4 DNA polymerase and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal3 (include Sal1 sequence). The cDNAs were purified by phenol/chloroform and separated from free linkers by Centricon 100. Then, cDNAs were amplified by long-range high fidelity PCR using Takara's Ex Taq polymerase. Then, the cDNAs were purified by phenol/chloroform and by Centricon 100. The cDNAs were digested with SalI and NotI enzymes. Then, the cDNAs were size selected by Gibco's Size Fractionation Column. The cDNAs were cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by chemical method. The library was constructed by Xiaohong Wang and Minoru S. H. Ko. |
| Name | Mouse four-cell-embryo cDNA |
| Organism | Mus musculus |
| Strain | C57BL/6J |
| Sex | Unknown |
| Stage | 4-cell stage embryo |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| Total RNAs were extracted 32 four-Cell stage embryos. The double-stranded cDNA was synthesized by Gibco's kit with an Oligo(dT) primer [NotI primer-adapter from GibcoBRL] [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 2.6µg of total RNA . The double-stranded cDNAs were treated with T4 DNA polymerase and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal3 (include Sal1 sequence). The cDNAs were purified by phenol/chloroform and separated from free linkers by Centricon 100. Then, cDNAs were amplified by long-range high fidelity PCR using Takara's Ex Taq polymerase. Then, the cDNAs were purified by phenol/chloroform and by Centricon 100. The cDNAs were digested with SalI and NotI enzymes. Then, the cDNAs were size selected by Gibco's Size Fractionation Column. The cDNAs were cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by chemical method. The library was constructed by Xiaohong Wang and Minoru S. H. Ko. |
| Name | Mouse eight-cell stage embryo cDNA |
| Organism | Mus musculus |
| Strain | C57BL/6J |
| Sex | Unknown |
| Stage | 8-cell stage embryo |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| Total RNAs were extracted from 230 eight-cell stage embryos. The double-stranded cDNA was synthesized by Gibco's kit with an Oligo(dT) primer [NotI primer-adapter from GibcoBRL] [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 4.3µg of total RNA . The double-stranded cDNAs were treated with T4 DNA polymerase and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal3 (include Sal1 sequence). The cDNAs were purified by phenol/chloroform and separated from free linkers by Centricon 100. Then, cDNAs were amplified by long-range high fidelity PCR using Takara's Ex Taq polymerase. Then, the cDNAs were purified by phenol/chloroform and by Centricon 100. The cDNAs were digested with SalI and NotI enzymes. Then, the cDNAs were size selected by Gibco's Size Fractionation Column. The cDNAs were cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by chemical method. The library was constructed by Xiaohong Wang and Minoru S. H. Ko. |
| Name | Mouse sixteen-cell stage embryo cDNA |
| Organism | Mus musculus |
| Strain | C57BL/6J |
| Sex | Unknown |
| Stage | 16-cell stage embryo |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| Total RNAs were extracted 42 sixteen-cell stage embryos. The double-stranded cDNA was synthesized by Gibco's kit with an Oligo(dT)GC primer [5' pGACTAGTTCTAGATCGCGAGCGGCCGCGCTTTTTTTTTTTTTTT-3'] from 2.1µg of total RNA . The double-stranded cDNAs were treated with T4 DNA polymerase and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal3 (include Sal1 sequence). The cDNAs were purified by phenol/chloroform and separated from free linkers by Centricon 100. Then, cDNAs were amplified by long-range high fidelity PCR using Takara's Ex Taq polymerase. Then, the cDNAs were purified by phenol/chloroform and by Centricon 100. The cDNAs were digested with SalI and NotI enzymes. Then, the cDNAs were size selected by Gibco's Size Fractionation Column. The cDNAs were cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by chemical method. The library was constructed by Xiaohong Wang and Minoru S. H. Ko. |
| Name | Mouse 3.5-dpc blastocyst cDNA |
| Organism | Mus musculus |
| Strain | C57BL/6J |
| Sex | Unknown |
| Stage | Mouse Blastocyst stage embryo |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| Total RNAs were extracted from 40 Blastocyst stage embryos. The double-stranded cDNA was synthesized by Gibco's kit with an Oligo(dT)-1 primer [5'GAGAGAGACTAGTTCTAGATCGCGAGCGGCCGCTTTTTTTTTTTTTTTTTT 3'] from 1.5µg of total RNA . The double-stranded cDNAs were treated with T4 DNA polymerase and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal3 (include Sal1 sequence). The cDNAs were purified by phenol/chloroform and separated from free linkers by Centricon 100. Then, cDNAs were amplified by long-range high fidelity PCR using Takara's Ex Taq polymerase. Then, the cDNAs were purified by phenol/chloroform and by Centricon 100. The cDNAs were digested with SalI and NotI enzymes. Then, the cDNAs were size selected by Gibco's Size Fractionation Column. The cDNAs were cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by chemical method. The library was constructed by Xiaohong Wang and Minoru S. H. Ko. |
| Name | NIA Mouse E7.5 Embryonic Portion cDNA Library |
| Organism | Mus musculus |
| Strain | C57BL/6J |
| Sex | Unknown |
| Stage | 7.5dpc Embryo |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| Total RNAs were extracted from 6 Embryo. The double-stranded cDNA was synthesized by Gibco's kit with an Oligo(dT) primer [NotI primer-adapter from GibcoBRL] [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 0.51µg of mRNA . The double-stranded cDNAs were treated with T4 DNA polymerase and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal3 (include Sal1 sequence). The cDNAs were purified by phenol/chloroform and separated from free linkers by Centricon 100. Then, cDNAs were amplified by long-range high fidelity PCR using Takara's Ex Taq polymerase. Then, the cDNAs were purified by phenol/chloroform and by Centricon 100. The cDNAs were digested with SalI and NotI enzymes. Then, the cDNAs were size selected by Gibco's Size Fractionation Column. The cDNAs were cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by chemical method. The library was constructed by Xiaohong Wang and Minoru S. H. Ko. |
| Name | NIA Mouse E7.5 Extraembryonic Portion cDNA Library |
| Organism | Mus musculus |
| Strain | C57BL/6J |
| Sex | Unknown |
| Stage | 7.5dpc Embryo |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| Total RNAs were extracted from 5 EPC. The double-stranded cDNA was synthesized by Gibco's kit with an Oligo(dT) primer [NotI primer-adapter from GibcoBRL] [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 0.8µg of mRNA . The double-stranded cDNAs were treated with T4 DNA polymerase and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal3 (include Sal1 sequence). The cDNAs were purified by phenol/chloroform and separated from free linkers by Centricon 100. Then, cDNAs were amplified by long-range high fidelity PCR using Takara's Ex Taq polymerase. Then, the cDNAs were purified by phenol/chloroform and by Centricon 100. The cDNAs were digested with SalI and NotI enzymes. Then, the cDNAs were size selected by Gibco's Size Fractionation Column. The cDNAs were cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by chemical method. The library was constructed by Xiaohong Wang and Minoru S. H. Ko. |
| Name | NIA Mouse E12.5 Female Mesonephros and Gonads cDNA Library |
| Organism | Mus musculus |
| Strain | C57BL/6J |
| Sex | Female |
| Stage | 12.5dpc |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | Plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| Total RNAs were extracted from 2 Mesonephros. The double-stranded cDNA was synthesized by Gibco's kit with an Oligo(dT) primer [NotI primer-adapter from GibcoBRL] [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 3.42µg of total RNA . The double-stranded cDNAs were treated with T4 DNA polymerase and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal3 (include Sal1 sequence). The cDNAs were purified by phenol/chloroform and separated from free linkers by Centricon 100. Then, cDNAs were amplified by long-range high fidelity PCR using Takara's Ex Taq polymerase. Then, the cDNAs were purified by phenol/chloroform and by Centricon 100. The cDNAs were digested with SalI and NotI enzymes. Then, the cDNAs were size selected by Gibco's Size Fractionation Column. The cDNAs were cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by chemical method. The library was constructed by Xiaohong Wang and Minoru S. H. Ko. |
| Name | NIA Mouse Newborn Ovary cDNA Library |
| Organism | Mus musculus |
| Strain | |
| Sex | Female |
| Stage | Newborn Ovary |
| Host | DH10B |
| Vector | pSPORT1 (Gibco/BRL Life Technology) |
| V_Type | plasmid (ampicillin resistant) |
| RE_1 | SalI |
| RE_2 | NotI |
| Description | |
| Total RNAs were extracted from 7 Newborn Ovary. The double-stranded cDNA was synthesized by Gibco's kit with an Oligo(dT) primer [NotI primer-adapter from GibcoBRL] [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 2.56µg of total RNA . The double-stranded cDNAs were treated with T4 DNA polymerase and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal3 (include Sal1 sequence). The cDNAs were purified by phenol/chloroform and separated from free linkers by Centricon 100. Then, cDNAs were amplified by long-range high fidelity PCR using Takara's Ex Taq polymerase. Then, the cDNAs were purified by phenol/chloroform and by Centricon 100. The cDNAs were digested with SalI and NotI enzymes. Then, the cDNAs were size selected by Gibco's Size Fractionation Column. The cDNAs were cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by chemical method. The library was constructed by Yu-Lan Piao and Minoru S. H. Ko. |
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